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Salmonella paratyphi B and Salmonella typhimurium were starved in seawater microcosms respectively for 12 and 6 years. After starvation periods, major part of water was evaporated in each microcosm and salt crystals appeared. No culturable cells were obtained in selective and non-selective media. Salty suspension of each microcosm was inoculated in the yolk sacs of embryonated chicken eggs. Culturable cells were detected in a large proportion of eggs.

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Recovery in embryonated chicken eggs of viable but non-culturable Salmonella

Abstract