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Author: Giedrė Dabkevičienė ^*, Vanda Paplauskienė and Izolda Pašakinskienė

Abstract

Hybrids were made between two species of clovers, Trifolium pratense L. (2n = 2x = 14) and Trifolium diffusum Ehrh. (2n = 2x = 16) and a T. pratense (♀) x [T. pratense x T. diffusum] backcross (BC₁) population was also developed. ISSR (inter-simple sequence repeats) markers were used to tag the hybrid plants. Traits of the BC₁s were compared with diploid and tetraploid Lithuanian cultivars of red clover. BC₁ plants were found to be of considerable agricultural value, despite the difficulties in transferring autogamy from T. diffusum into the allogamous T. pratense. Improved seed yield in BC₁ plants was achieved due to a significant increase of the number of inflorescences per plant in comparison with diploid red clover. Some of T. pratense x T. diffusum hybrids expressed high levels of autogamy which provides a valuable resource for studies on the inheritance of autogamy.

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Assessment of the agronomic utility of interspecific hybrids Trifolium pratense L. x T. diffusum Ehrh. and confirmation of their hybridity with ISSR markers

Abstract

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